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The Caloosahatchee National Wildlife Refuge is part of the United States National Wildlife Refuge System, located on the Caloosahatchee River, beneath the I-75 Caloosahatchee Bridge, within the city of North Fort Myers. The 40-acre (160,000 m2) refuge was established on January 1, 1921. It is administered as part of the J. N. "Ding" Darling National Wildlife Refuge Complex.

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